The Disney+ platform also reduced its losses by $400 million (£316.5 million).
As the traditional film and television market shrinks, the studio behind Mickey Mouse, the Star Wars series, and the Marvel movies is under pressure to turn its streaming business profitable.
In New York's after-hours trading, the company's shares decreased by about 5%.
The Hotstar service in Asia, which lost the ability to stream Indian cricket matches last year, accounted for the majority of the subscriber losses. In addition, Disney+ lost about 300,000 subscribers in the US and Canada as a result of increasing subscription costs.
It occurs as operating losses for Disney's streaming division have decreased.
It happens at a time when Disney's streaming division has cut its operating losses for the first three months of this year to $659 million. From $1.1bn the previous quarter, that amount decreased.
The company's improved financial performance, according to Disney CEO Bob Iger, "reflects the strategic changes we've been making throughout the organization to realign Disney for sustained growth and success."
A year ago, he predicted Disney+ had reached a "turning point" and would start to turn a profit.
The major entertainment company reported its first decline in subscriber numbers earlier this year and revealed plans to lay off 7,000 employees.
After staging their first strike in fifteen years last week, thousands of Hollywood screenwriters for TV and movies made the most recent announcement.
As the traditional television and film industries have been drastically altered by the shift to streaming, they are requesting better pay and working conditions.
2007 saw the most recent writers' strike. It cost the industry an estimated $2 billion and lasted 100 days.
Christine McCarthy, the chief financial officer of Disney, declined on Wednesday to estimate how much the most recent strike might cost the business.
Numerous Disney initiatives, including those scheduled to air on Disney+, have already been put on hold due to the walkout.
Disney, a company with roots in traditional television, movies, and theme parks, has invested billions of dollars in its streaming platforms in recent years, making it one of the major players in the streaming market.
Over 231 million people have subscribed to its three streaming services, which also include the sports-focused ESPN+ and the more general entertainment website Hulu.
Disney+ has nearly 158 million subscribers worldwide, but that number still lags behind Netflix, which has 232.5 million.
